Biggar will finish his 18-year career with French club Toulon, who he joined in November 2022.
The 35-year-old won 112 caps for Wales in a 15-year Test career and scored more than 600 points. His final appearance was at the 2023 Rugby World Cup.
A winner of three Six Nations titles and a Grand Slam with Wales, Biggar was also captain during the Wayne Pivac era, whilst also appearing on two British and Irish Lions tours, making three Test appearances in South Africa in 2021.
“There comes a point where you just know, not because of anything in particular, but someone once said to me ‘when you know, you’ll know’,” said Biggar on a video posted social media platform Instagram.
“Rugby has given me everything. I threw myself into this game at 17 and it’s given me a life that I could never have imagined.
“I’ve lived out my childhood dreams for the best part of two decades and I’m so, so grateful for that.”
Biggar started his youth career at Gorseinon RFC and Gowerton Comprehensive in Swansea before playing a season for Swansea RFC in the Welsh Premiership.
He made his senior Ospreys debut in 2008 and during an 11-year spell made 221 appearances and scored 2,203 points, making him the region’s record points scorer and was also instrumental in securing two Pro12 titles in 2010 and 2012.
“There are a few moments I will never forget – whether it’s my first cap for Wales, becoming a Test centurion and a Test Lion are extremely special.
“But the thing I am most proud about is being able to give my family opportunities in life. That’s what means the most to me.
“It certainly hasn’t always been plain-sailing and easy. Playing number 10 for Wales brought its own unique pressure and challenges, but it made me stronger as a person and a player.
“I also know that the last 12 months haven’t quite gone as I had hoped with Toulon. It has been tough, and setbacks teach you a lot more about yourself.
“There is no real perfect moment to walk away, but this does feel like the right one. I am choosing to retire, which a lot of sportspeople don’t get to do, when they are healthy, and I feel very grateful.”
[Lead image: Welsh Rugby Union]